Mutant Ataxin‐3 inhibits 3’ phosphatase activity of human polynucleotide kinase 3′‐phosphatase (PNKP)
نویسندگان
چکیده
منابع مشابه
Polynucleotide kinase from a T4 mutant which lacks the 3' phosphatase activity.
Polynucleotide kinase from E. coli infected with the PseT 1 mutant of bacteriophage T4 has been isolated. The PseT 1 enzyme purifies similarly to normal polynucleotide kinase and effectively transfers the gamma phosphate of ATP to the 5' terminal hydroxyl of DNA and RNA. The PseT 1 and normal enzymes require similar magnesium ion concentrations, have the same pH optima and are both inhibited by...
متن کاملMolecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.
Mammalian polynucleotide kinases catalyze the 5'-phosphorylation of nucleic acids and can have associated 3'-phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. The sequences of three tryptic peptides from a bovine 60-kDa polypeptide that correlated with 5'-DNA kinase and 3'-phosphatase activities identified human and murine ...
متن کاملStructure-function analysis of the 3' phosphatase component of T4 polynucleotide kinase/phosphatase.
T4 polynucleotide kinase/phosphatase (Pnkp) exemplifies a family of bifunctional enzymes with 5'-kinase and 3' phosphatase activities that function in nucleic acid repair. T4 Pnkp is a homotetramer of a 301-aa polypeptide, which consists of an N-terminal kinase domain of the P-loop phosphotransferase superfamily and a C-terminal phosphatase domain of the DxD acylphosphatase superfamily. The hom...
متن کاملDNA end processing by polynucleotide kinase/phosphatase.
S cission of one or both strands of the DNA duplex occurs as ionizing radiation and oxidative DNA damage mount a constant assault our genomes. Like a broken limb, or split piece of wood, DNA strand breaks are typically not clean. That is, they lack the DNA 3′-hydroxyl and 5′-phosphate moieties needed for DNA repair synthesis by DNA polymerases and DNA end joining by DNA ligases (Fig. 1A). For i...
متن کاملMechanism of RNA 2′,3′-cyclic phosphate end healing by T4 polynucleotide kinase–phosphatase
T4 polynucleotide kinase-phosphatase (Pnkp) exemplifies a family of enzymes with 5'-kinase and 3'-phosphatase activities that function in nucleic acid repair. The polynucleotide 3'-phosphatase reaction is executed by the Pnkp C-terminal domain, which belongs to the DxDxT acylphosphatase superfamily. The 3'-phosphatase reaction entails formation and hydrolysis of a covalent enzyme-(Asp165)-phosp...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2013
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.27.1_supplement.lb79